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shown in Figure P312 and determine the voltage across R4 Note that one source is a controlled voltage source! VS = 5 V R2 = 18 k AV = 70 R3 = 68 k R1 = 22 k R4 = 220

R1 = 15

Cotransduction Frequencies in the Experiment Used to Determine the A B C Gene Order (Table 7.6)

R2 = 40

36 37

316 Using mesh current analysis, nd the voltage, v,

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Class A B C A B C A B C A B C Relative Cotransductance A-B: (50 75)/426 0.29 A-C: (50 1)/426 0.12 Number 50 75 1 300 426

2 1 3

Table 7.7 also includes calculations of the relative cotransduction frequencies. Remember that in all organisms and viruses, the higher the frequency of cooccurrence between the alleles of two loci, the closer those loci are on the chromosome. We usually measure the separation of loci by crossing over between them; the closer together, the lower those crossing-over values are and, hence, the smaller the measure of map units

313 Using mesh current analysis, nd the voltage, v,

317 Using mesh current analysis, nd the current, i,

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4 Advances in Precision Grinding 41 Introduction 42 Grinding Wheel 421 Bonding Materials 422 Abrasive Types 423 Grit Size 424 Grade 425 Structure 426 Concentration 427 Design and Selection of the Grinding Wheel 428 Mounted Wheels 429 Bondless Diamond Grinding Wheel 43 Conventional Grinding 44 Precision Grinding Processes 441 Jig Grinding for IC Chip Manufacturing 442 Precision Grinding with Electrolytic In-process Dressing (ELID) 443 Various Methods for Generating an Aspheric Surface 45 Ultra-Precision Grinding 451 Various Ultra-precision Machines and Their Development 452 Some Applications of Ultra-precision Machining

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apart. Here, as with transformation, we are measuring the co-occurrence directly; therefore, the measure cotransductance is the inverse of map distance. In other words, the greater the cotransduction rate, the closer the two loci are; the more frequently two loci are transduced together, the closer they are and the higher the cotransduction value will be. The data in table 7.7 should not be used to calculate the B-C cotransduction rate because the data are selected values, all of which are A ; they do not encompass the total data. Missing is the A B C group that would contribute to the B-C cotransductance rate. The A B C and A B C groups, also missing, would contribute only to the totals in the denominator, not the numerator, of the cotransductance index. From these sorts of transduction experiments, it is possible to round out the details of map relations in E. coli after obtaining the overall picture by interrupted mating. The partial map of E. coli appears in gure 7.27. De nitions of loci can be found in table 7.8. Unlike the measurements in eukaryotic mapping, prokaryotic map distances are not generally thought of in map units (centimorgans). Rather, the general distance between loci is determined in minutes with cotransduction values used for loci that are very close to each other. (In chapter 13, we discuss mapping methods that rely on directly sequencing the DNA.)

through the voltage source in the circuit of Figure P35 318 Using mesh current analysis, nd the current, i, in the circuit of Figure P36 319 Using mesh current analysis, nd the equivalent resistance, R = v/i, seen by the source of the circuit in Figure P319

short circuit However, if excess current ows through a fuse, its element melts and the fuse blows, ie, it becomes an open circuit VS1 = VS2 = 115 V R1 = R2 = 5 R3 = 10 R4 = R5 = 200 m Normally, the voltages across R1 , R2 , and R3 are 1065 V, 1065 V, and 2130 V If F1 now blows, or opens, determine, using KCL and a node analysis, the new voltages across R1 , R2 , and R3

0 10

1 i + v 1/4 1/5

map of E. coli. De nitions of loci not found in the text can be found in table 7.8. Units on the map are in minutes. Arrows within the circle refer to Hfr-strain transfer starting points, with directions indicated. The two thin regions on the outer circle are the only areas not covered by P1 transducing phages. (From B. J.

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