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and dignity (Hermann, 1987, p 167) In Millon s evolutionary terms, the motivating aim of nationalism clearly is a lifepreserving (pain-avoidant) orientation The pain pleasure dimension also provides evolutionary underpinnings for Barber s (1972/1992) fourfold (active passive positive negative) categorization of presidential character, in which positivity negativity is described in terms of enjoyment derived from political of ce Positive leaders have a generally optimistic outlook and derive pleasure from the duties of public of ce, whereas negative leadership has a more pessimistic tone, being oriented toward pain aversion Finally, the pain pleasure dimension suggests a possible evolutionary basis for the three management models proposed by Johnson (1974) and employed by George and Stern (1998) to classify the policy-making structures and advisory systems favored by recent US presidents Formalistic chief executives prefer an orderly policymaking [sic] structure, .

well-de ned procedures, hierarchical lines of communication, and a structured staff system (George & Stern, 1998, p 203) In evolutionary terms, they seek to preserve life by minimizing pain Competitive chief executives encourage more open and uninhibited expression of diverse opinions, analysis, and advice and tolerate or encourage organizational ambiguity, overlapping jurisdictions, and multiple channels of communication to and from the president (George & Stern, 1998, p 203) In evolutionary terms, they seek to enhance life by maximizing pleasure Collegial chief executives attempt to bene t from the advantages of both the competitive and formalistic approaches while avoiding their pitfalls Thus, they strive for diversity and competition in the policymaking system, balanced by encouraging cabinet of cers and advisers to identify at least partly with the presidential perspective and encouraging collegial participation (George & Stern, 1998, p 203).

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force after internal temperature reach 70 (Ritchey and Hostetler 1965; Bouton et al. 1976, 1982; Leander et al. 1980). However, other researchers have reported a decrease in shear force values at temperatures between 50 C and 60 C (Davey and Neiderer 1977; Hearne et al. 1978). The different observations could be associated with temperatures being evaluated. Bouton et al. (1976) reported

In evolutionary terms, collegial executives are intermediate on both the pleasure-seeking and pain-avoidant polarities The systematic import of a generative theory is implicit in the suggestion that Johnson s (1974) management model fails to account for at least two additional executive styles: complex types high on both the pleasure-seeking and pain-avoidant polarities, and undifferentiated types low on both valences Modes of Adaptation: The Passive Active Polarity The passive active polarity (Millon, 1990, pp 64 77; Millon, this volume) is conceptualized in terms of ecologic modi cation (active) and ecologic accommodation (passive); that is, whether initiative is taken in altering and shaping life s events or whether behaviors are reactive to and accommodate those events (Millon, this volume)..

2. Enter the number of elements in the array. Remember that the first element of an array is 0. If you re creating an array with 20 elements, enter 19. Listing 8-7 shows the code for an associative array with 20 elements.

Just click the Toolbox button that corresponds to the control you want to add, and then click inside the dialog box to create the control (using its default size). Or, you can click the control and then drag in the dialog box to specify the dimensions for the control. When you add a new control, it is assigned a name that combines the control type with the numeric sequence for that type of control. For example, if you add a CommandButton control to an empty UserForm, it is named CommandButton1. If you then add a second CommandButton, it is named CommandButton2.

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Department of Chemical Engineering1 , and Division of Biological Engineering2 , Massachusetts Institute of Technology, 77 Massachusetts Avenue, E25-342, Cambridge, Massachusetts 02139 Corresponding author: rlanger@mitedu 1 2 Embryonic Stem Cells Maintenance and Expansion of Human Embryonic Stem (HES) Cells 21 Preparation of Media and Reagents 211 Stock Solutions 212 Murine Embryonic Fibroblast (MEF) Medium 213 ES Cell Medium 214 Embryoid Body (EB) Cell Media 22 Preparation of MEF Cells Protocol 31 Seeding Cryopreserved Murine Embryonic Fibroblast (MEF) Cells Protocol 32 Seeding Mitotically Inactivated Murine Embryonic Fibroblasts (MEF) for hES Culture 23 ES Cell Expansion and Passaging Protocol 33 Passaging Human Embryonic Stem Cells (hES) with Collagenase 62 63 64 64 65 65 65 66 66 66 67 67.

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