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(e) Add 10 mL of growth medium and gently pipette cell suspension up and down to break up any remaining cell clumps and produce a single-cell suspension. (f) Split cell suspension equally 1:4 between 4 fresh asks [of the same size used in Step (b) above] and culture in growth medium without retinoic acid for a further 4 6 days (Stage A2). During this time, neurons are readily identi ed above a background of at nonneuronal cells (Fig. 6.6). (g) Remove growth medium from Stage A2 cells, add 1 mL of trypsin/EDTA solution, and incubate for 2 min at room temperature. (h) Detach cells loosely attached to the surface of the culture by applying a lateral motion provided by ve short, sharp blows with the palm of the hand to the side of the horizontal tissue culture ask. (i) Collect cells displaced by enzymatic/mechanical disruption in 10 mL of growth medium. (j) Combine cell suspensions from more than one ask, determine cell number, and wash and resuspend pellet in growth medium. (k) Seed cell suspensions at 2 105 cells/cm2 (Stage A3) and culture in growth medium containing either: (i) 1 M cytosine arabinoside (for the rst 10 days only), 10 M uorodeoxyuridine, 10 M uridine, to enhance the purity of neurons and reduce the proliferation of nonneuronal cell types (Fig. 6.7) or (ii) 0.1 M cytosine arabinoside (for the rst 10 days only), 3 M uorodeoxyuridine, 5 M uridine, to allow limited proliferation of astrocytic glial cells.

Functions in PHP can have a default argument assigned, which means they won t require that argument to be passed in, such as:

A revolutionary advancement in modern medicine was the discovery of antibiotics compounds that can be taken orally or intravenously by humans to destroy microbial agents of disease. Using the lexicon from werewolf folklore, antibiotics are silver bullets able to act selectively on target organisms, while sparing the infected host. The antibiotic

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The regular expression-specific special characters, \n (where n is an integer from 1 to 9), \b, \B, \d, \D, \s, \S, \w, and \W, should all appear in your ActionScript code with double slashes in front of them (for example, \\b\\w ). However, because many of the special characters (\f, \r, \n, \t, \v) are already recognized by Flash, these characters should not appear with double slashes. Instead, they should appear with single slashes (for example, \f\n ). You will see examples of this in the code examples throughout this chapter.

In order for Dreamweaver to properly process an HTML file using any browser profile, the profile must follow a precise format. Here s an excerpt from the Internet Explorer 6.0 browser profile:

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30: Localizing Flex 4 Applications .................................................................................941 31: Deploying Desktop Applications with Adobe AIR....................................................955

A combination chart is a single chart that consists of series that use different chart types. A combination chart may also include a second value axis. For example, you may have a chart that shows both columns and lines, with two value axes. The value axis for the columns is on the left, and the value axis for the line is on the right. A combination chart requires at least two data series. Creating a combination chart simply involves changing one or more of the data series to a different chart type. Select the data series to change and then choose Chart Tools Design Type Change Chart Type. In the Change Chart Type dialog box, select the chart type that you want to apply to the selected series. Using a second Value Axis is optional.

Purpose: Calculates the payment needed to fully amortize a loan, given the loan amount, the interest rate per payment period, and the number of periods. Syntax: PMT (principal; rate; periods) where principal is a number representing the amount of the loan, rate is the interest rate per period, and periods is the number of payments. Example: The following expression

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Figure 30-2: When you re creating your selection, pay particular attention to the edges of the rooftops.

n InDesign scripting documentation: The InDesign installation CD contains a 2,000-pluspage PDF file that explains scripting for InDesign. This document, although a bit on the technical side, is a valuable resource. It includes an overview of JavaScript scripting and the object model, as well as a list of InDesign-specific scripting terms and scripting examples. If you want still more information about JavaScript, several books are available, including Beginning JavaScript, 2nd Edition, by Paul Wilton and JavaScript Bible, 6th Edition, by Danny Goodman and Michael Morrison (both from Wiley Publishing).

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